Von Teese has appeared on a number of best-dressed lists and frequents the front row of fashion shows, particularly Christian Dior, Vivienne Westwood, Jean Paul Gaultier and Marc Jacobs, labels she is often seen wearing. Speaking of her love of Gaultier, Von Teese has said, "Jean Paul Gaultier, John Galliano and Vivienne Westwood were the first designers that really made an impact on me. Jean Paul has a lot of the same obsessions that I do, like corsetry, ballet-peach satin and silks, black silk velvet, bullet bras and girdles. When I was a teenager, I would look for 1930s peach satin lingerie pieces to try to get his look for less, because there was no way I could afford his things back then. It was a dream come true to meet him and become friends with him. I met him the first time when I was doing my first fashion pictorial, which was for Flaunt magazine, and it was a huge story of me wearing the most important pieces from his haute couture archives, and that was a dream!" 
She has also done catwalk work. During Los Angeles Fashion Week for spring 2004, she modeled for former club kid Richie Rich's fashion label, Heatherette. In 2005 she appeared in the Autumn/Winter Ready-to-Wear show for Giambattista Valli, a former designer for Ungaro, in Paris. In the 2006 Milan Fashion Week, Von Teese was on the runway, opening for the Moschino diffusion label, Moschino Cheap & Chic, autumn/winter 2006/7 show. In 2007, she appeared twice in the Jean Paul Gaultier haute couture show during the Paris Fashion Week. She has starred in several ad campaigns. She appeared in Vivienne Westwood's spring/summer 2005 collection adverts and became the face of Australian clothing range "Wheels and Dollbaby" for their 2006/7 Spring/Summer advertising campaign. She was an ambassador/spokesmodel for HIV/AIDS awareness when she was selected (along with Eve, Deborah Harry, and Lisa Marie Presley) for MAC Cosmetics' Viva Glam VI campaign (a collection of lipsticks and lipgloss where 100% of the proceeds are for worldwide AIDS charities and to raise awareness for HIV/AIDS). Von Teese has appeared in Vanity Fair, Vogue, Elle, and international issues of nearly every fashion magazine. She has campaigned on behalf of People for the Ethical Treatment of Animals (PETA) and has appeared in their ads. She participated at the PETA's campaign "Animal Birth Control (ABC)."
In spite of this, Von Teese states that she never uses a stylist. "The one time I hired a stylist, they picked up a pair of my 1940s shoes and said, 'These would look really cute with jeans.' I immediately said, 'You’re out of here.'" She does her own make-up, and dyes her naturally blonde hair black at home. Von Teese's unique style is "inspired by eccentric women like Luisa Casati, Anna Piaggi, and Isabella Blow."
She has designed a new lingerie range with Wonderbra. In May 2012, Dita Von Teese launched her makeup collection "Classics" in partnership with the German cosmetic brand Art Deco. Compact powder, blushers, eye styler, eye shadows, mascara and lipstick were produced to recreate the burlesque performer's retro look.
In 2012 Dita Von Teese launched her own clothing line and underwear line called Von Follies. In 2013 Dita was both model and muse for the 'Dita' 3D printed gown designed by Michael Schmidt and Francis Bitonti and 3D printed by Shapeways exactly to fit her body. Constructed of over 3,000 interlocked 3D printed components and over 12,000 Swarovski crystals it is one of the first fully articulated 3D printed garments to take the technology from haute couture to a sensual fabric-like form.
In 2014, Von Teese began working with Myer, an Australian department store, on a lingerie line fronted by Australian model Stefania Ferrario.